Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

Applications include self-circularization of linear DNA prior to transformation, ligation of insert to vector, and ligation of synthetic linkers to blunt-ended DNA. Each predispensed, single-reaction, ambient-temperature-stable tube contains a glassified reaction mix which, when reconstituted to a final reaction volume of 20 μL, yields a solution of reaction buffer, T4 DNA ligase, and ATP.

Features and Benefits

•For covalently-linking DNA fragments in as little as 30 min.•Single-dose, ambient-temperature-stable reaction mixes.

General description

Ready-To-Go^™ T4 DNA Ligase. Catalyzes the formation of a phosphodiester bond between the 5′-phosphoryl group and the 3′-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction. Ligation of blunt-ended DNA is greatly stimulated by polyethylene glycol.

Legal Information

Ready-To-Go is a trademark of Cytiva